SETD8 Polyclonal Antibody for Human(Homo sapiens) | EIAab.comSETD8 Polyclonal Antibody for Human(Homo sapiens)

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SETD8 (Gene name),

N-lysine methyltransferase SETD8 (Protein name ), SETD8_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

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Gene name:

SETD8(KMT5A;PRSET7;SET07;SET8);

Protein name:

N-lysine methyltransferase SETD8;

Alternative:

2.1.1.43;Histone-lysine N-methyltransferase SETD8);H4-K20-HMTase SETD8;PR/SET domain-containing protein 07(PR-Set7;PR/SET07);Lysine N-methyltransferase 5A;SET domain-containing protein 8;

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Interacts with L3MBTL1.

Function:

Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes.

Subcellular Location:

Nucleus Chromosome Specifically localizes to mitotic chromosomes. Associates with silent chromatin on euchromatic arms. Not associated with constitutive heterochromatin.

Protein Attributes:

Sequence length:

393

Sequence:

50:

100:

150:

200:

250:

300:

350:

393:

HDIDGVPHLI | LIASRDIAAG | EELLYDYGDR | SKASIEAHPW | LKH

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

STRING:

9606.ENSP00000332995

Pfam:

PF00856

UniGene:

Hs.443735

SMR:

Q9NQR1

KEGG:

hsa:387893

MIM:

607240

String:

Q9NQR1

Uniprot:

Q9NQR1

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ELISA Kit for Human N-lysine methyltransferase SETD8

Cat.:

E3859b

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Packing:

96T

ELISA Kit for Human N-lysine methyltransferase SETD8

Cat.:

E3859m

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96T

ELISA Kit for Human N-lysine methyltransferase SETD8

Cat.:

E3859h

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96T

CLIA Kit for Human N-lysine methyltransferase SETD8

Cat.:

U3859m

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MSDS:

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Packing:

96T

CLIA Kit for Human N-lysine methyltransferase SETD8

Cat.:

U3859b

Price:

Please sign in first.

MSDS:

Please sign in first.

Packing:

96T

CLIA Kit for Human N-lysine methyltransferase SETD8

Cat.:

U3859h

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96T

Polyclonal Antibody for Human N-lysine methyltransferase SETD8

Cat.:

P3859Rb-h

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human N-lysine methyltransferase SETD8

Cat.:

P3859Rb-m

Price:

Please sign in first.

Packing:

40ug/0.2ml

Polyclonal Antibody for Human N-lysine methyltransferase SETD8

Monoclonal Antibody for Human N-lysine methyltransferase SETD8

Protein for Human N-lysine methyltransferase SETD8

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

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Recovery

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Linearity

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References

1.

"PR-Set7 is a nucleosome-specific methyltransferase that modifies lysine 20 of histone H4 and is associated with silent chromatin."
Nishioka K. , Rice J.C. , Sarma K. , Erdjument-Bromage H. , Werner J. , Wang Y. , Chuikov S. , Valenzuela P. , Tempst P. , Steward R. , Lis J.T. , Allis C.D. , Reinberg D.
Mol. Cell9:1201-1213(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);PROTEIN SEQUENCE OF 108-131; 220-231 AND 349-393;FUNCTION;SUBCELLULAR LOCATION;CATALYTIC ACTIVITY;MUTAGENESIS OF ARG-336
tissue: Cervix carcinoma.
2.

"Purification and functional characterization of SET8, a nucleosomal histone H4-lysine 20-specific methyltransferase."
Fang J. , Feng Q. , Ketel C.S. , Wang H. , Cao R. , Xia L. , Erdjument-Bromage H. , Tempst P. , Simon J.A. , Zhang Y.
Curr. Biol.12:1086-1099(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2);PROTEIN SEQUENCE OF 83-103; 109-134; 141-151; 162-172; 221-230; 245-260; 280-297 AND 350-393;FUNCTION;CATALYTIC ACTIVITY;MUTAGENESIS OF HIS-340 AND 385-ILE--HIS-393
3.

"A novel PR/SET domain-containing gene, SET07, as a candidate tumor suppressor."
Tain F. , Huang S.
Submitted (2001-07) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
4.

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2)
tissue: Thymus.
5.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2)
tissue: Testis.
6.

"Mitotic-specific methylation of histone H4 Lys 20 follows increased PR-Set7 expression and its localization to mitotic chromosomes."
Rice J.C. , Nishioka K. , Sarma K. , Steward R. , Reinberg D. , Allis C.D.
Genes Dev.16:2225-2230(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION;DEVELOPMENTAL STAGE
7.

"A switch in mitotic histone H4 lysine 20 methylation status is linked to M phase defects upon loss of HCF-1."
Julien E. , Herr W.
Mol. Cell14:713-725(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INDUCTION
8.

"SET8 recognizes the sequence RHRK20VLRDN within the N terminus of histone H4 and mono-methylates lysine 20."
Yin Y. , Liu C. , Tsai S.N. , Zhou B. , Ngai S.M. , Zhu G.
J. Biol. Chem.280:30025-30031(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: CATALYTIC ACTIVITY
9.

"A trans-tail histone code defined by monomethylated H4 Lys-20 and H3 Lys-9 demarcates distinct regions of silent chromatin."
Sims J.K. , Houston S.I. , Magazinnik T. , Rice J.C.
J. Biol. Chem.281:12760-12766(2006) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
10.

"Modulation of p53 function by SET8-mediated methylation at lysine 382."
Shi X. , Kachirskaia I. , Yamaguchi H. , West L.E. , Wen H. , Wang E.W. , Dutta S. , Appella E. , Gozani O.
Mol. Cell27:636-646(2007) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;MUTAGENESIS OF ASP-379
11.

"Histone H4 lysine 20 monomethylation promotes transcriptional repression by L3MBTL1."
Kalakonda N. , Fischle W. , Boccuni P. , Gurvich N. , Hoya-Arias R. , Zhao X. , Miyata Y. , Macgrogan D. , Zhang J. , Sims J.K. , Rice J.C. , Nimer S.D.
Oncogene27:4293-4304(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH L3MBTL1
12.

"A quantitative atlas of mitotic phosphorylation."
Dephoure N. , Zhou C. , Villen J. , Beausoleil S.A. , Bakalarski C.E. , Elledge S.J. , Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A.105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-100;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
13.

"The MBT repeats of L3MBTL1 link SET8-mediated p53 methylation at lysine 382 to target gene repression."
West L.E. , Roy S. , Lachmi-Weiner K. , Hayashi R. , Shi X. , Appella E. , Kutateladze T.G. , Gozani O.
J. Biol. Chem.285:37725-37732(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: MUTAGENESIS OF ASP-379
14.

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V. , Vermeulen M. , Santamaria A. , Kumar C. , Miller M.L. , Jensen L.J. , Gnad F. , Cox J. , Jensen T.S. , Nigg E.A. , Brunak S. , Mann M.
Sci. Signal.3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-100;IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]
tissue: Cervix carcinoma.
15.

"The tumor suppressor SirT2 regulates cell cycle progression and genome stability by modulating the mitotic deposition of H4K20 methylation."
Serrano L. , Martinez-Redondo P. , Marazuela-Duque A. , Vazquez B.N. , Dooley S.J. , Voigt P. , Beck D.B. , Kane-Goldsmith N. , Tong Q. , Rabanal R.M. , Fondevila D. , Munoz P. , Kruger M. , Tischfield J.A. , Vaquero A.
Genes Dev.27:639-653(2013) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: ACETYLATION AT LYS-172;DEACETYLATION AT LYS-172 BY SIRT2;INTERACTION WITH SIRT2;SUBCELLULAR LOCATION;MUTAGENESIS OF LYS-172;MASS SPECTROMETRY (ISOFORM 2)
16.

"CRL1-FBXO11 promotes Cdt2 ubiquitylation and degradation and regulates Pr-Set7/Set8-mediated cellular migration."
Abbas T. , Mueller A.C. , Shibata E. , Keaton M. , Rossi M. , Dutta A.
Mol. Cell49:1147-1158(2013) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;INDUCTION;UBIQUITINATION;MUTAGENESIS OF ARG-336 AND ASP-379
17.

"Specificity and mechanism of the histone methyltransferase Pr-Set7."
Xiao B. , Jing C. , Kelly G. , Walker P.A. , Muskett F.W. , Frenkiel T.A. , Martin S.R. , Sarma K. , Reinberg D. , Gamblin S.J. , Wilson J.R.
Genes Dev.19:1444-1454(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 233-393 IN COMPLEX WITH HISTONE H4 AND S-ADENOSYLMETHIONINE;FUNCTION
18.

"Structural and functional analysis of SET8, a histone H4 'Lys-20' methyltransferase."
Couture J.-F. , Collazo E. , Brunzelle J.S. , Trievel R.C.
Genes Dev.19:1455-1465(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (1.45 ANGSTROMS) OF 231-393 IN COMPLEX WITH HISTONE H4 AND S-ADENOSYLMETHIONINE;FUNCTION;MUTAGENESIS OF TYR-286; GLU-300; CYS-311; TYR-375; ASP-379 AND HIS-388

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